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Zebrafish and mouse husbandry and treatment Zebrafish wild type (WT) lines used in this work, including those used for the generation of the stable mutant lines, derived from Tuebingen and Giotto strains matings

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or long-acting testosterone undecanoate / Nebido every 1014 weeks) Transdermal gels or creams applied daily to the skin Patches worn on the skin Subcutaneous pellets implanted under the skin every few months (less common in Thailand) Because you are adding testosterone from outside, your brain detects "enough testosterone" and dials down its own signal to the testes