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After supplementation with 10% glycerol, aliquots of purified protein were flash-frozen in liquid nitrogen and stored at 80 C until further use

doi: 10.4103/0974-7753.114706 [DOI] [PMC free article] [PubMed] [Google Scholar] 66.Anggraini DR, Feriyawati L

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17-HSD) 3-hydroxysteroid dehydrogenase (3-HSD) and 17-hydroxysteroid dehydrogenase (17-HSD) in cytosolic fraction were assessed photometrically following the reduction of NAD to NADH at 399 nm at 25C, pH 8.9