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Unpaired t test was performed using GraphPad Prism v9.4.0.673 for Windows (GraphPad Software, San Diego, California USA)

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27 The major limitations of this study are the lack of verifiable diagnoses reported by respondents, lack of objective symptom improvement, and low survey response rate, all of which were anticipated weaknesses given the study design

Furthermore, studies have reported an aberrant activation of NRF2, a key antioxidant transcription factor involved in the regulation of critical antioxidants including glutathione, SOD, and CAT, leading to reduced antioxidant capacity (Hirayama et al., 2020)