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Reverse transcription was applied at 50 C for 30 min, a primary denaturation step was done at 95 C for 5 min, followed by 35 cycles of 94 C for 30 s., annealing (55 C for 40 s for bovine viral diarrhea virus)

The GD group exhibited higher levels of serum and muscle antioxidant enzymes along with upregulated expression of Nrf2 and its downstream targets (HO-1, NQO1) 24

2.4 ALKBH5-PRMT2- -catenin-GPX4 signaling pathway The ALKBH5-PRMT2--catenin-GPX4 axis is believed to play an important role in promoting LPS (lipopolysaccharide)-induced ferroptosis in microglial cells, providing new insights into the pathogenesis of depression