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Quantitative LCMS measurements were also performed in positive ion mode using a Triple Quadrupole (QQQ) mass spectrometer (Applied Biosystems, API 3000 QQQ) equipped with an electrospray ion source (ESI), and combined with a Shimadzu High Performance LC system (CBM-20 A, binary pump LC-20AB)

When this happens, low levels of intrinsic factor produce symptoms of juvenile pernicious anemia in patients younger than age 10

Though historically considered weak compared to C3a and C5a, C4a exerts direct biological effects including endothelial cell activation, increased vascular permeability, and mast cellindependent smooth muscle contraction