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The liver tissues were homogenized using a Tris-HCL buffer at a ratio of 1:5 (liver tissue to buffer) with a concentration of 150 mM and a pH of 7.4


RAGE is a transmembrane receptor and is expressed in numerous tissues including ovaries, heart, lung and skeletal muscle, but also in monocytes, macrophages and lymphocytes [41]

In view of the large number of methods that have been reported for attaching a variety of radiodiagnostic compounds, radiotherapeutic compounds, drugs, toxins, and other agents to antibodies one skilled in the art will be able to determine a suitable method for attaching a given agent to an antibody or other polypeptide
