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Description
Importantly, LDHA was identified among the top upregulated factors observed in the proteomic profile of gastric cancer patients resistant to platinum-based neoadjuvant chemotherapy
In brief, 0.5 ml of the supernatant of brain homogenate was added to exactly 4.5 ml of working reagent (1 volume from 0.8% thiobarbituric acid + 3 volume from 20% trichloroacetic acid)
Add proper amount of pre-cooled acetone to wash, repeat 3 times
It is stable at -20C for 3 months after opening
