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2-Oxoaldehydes are harmful to cells since they react with guanidine residues in nucleic acids as well as with arginine, lysine and cysteine moieties in proteins

Lysate concentration was determined using Bradford assay (BSA standard) and diluted to 1 mg ml 1 and incubated with tetrazine-Cy5 (1 M, 30 minutes, 37 C) and then quenched with 5 l of 4 Laemmeli dye containing 6% -mercaptoethanol

Small molecule proteostasis regulators that reprogram the ER to reduce extracellular protein aggregation

The first day of the 2 protocols was considered an adjustment period, and included the patients history and physical, blood work, and baseline studies