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3 Multi-omics insights guiding microbiome engineering Recent multi-omics advances clarify hostmicrobiomeenvironment interactions by resolving microbial composition, function, and host responses at high resolution
Then, membranes were incubated with primary antibodies against NOS2 (1:1000, ABclonal, Wuhan, China), NOX1 (1:1000, ABclonal, Wuhan, China), HO-1 (1:1000, ABclonal, Wuhan, China), SOD2 (1:1000, ABclonal, Wuhan, China), Kelch-like ECH-associated protein 1 (KEAP1) (1:1000, Proteintech, Wuhan, China), nuclear factor B (NF-B) P65 (1:1000, Cell Signaling Technology, Danvers, USA), GAPDH (1:10000, Proteintech, Wuhan, China), Histone H3 (1:4000, HUABIO, Hangzhou, China) and Tublin- (1:10000, abcam, USA) at 4 C overnight followed by incubation with secondary antibody (1:5000, Proteintech, Wuhan, China) for 1 h at RT

Moreover, SFN through the formation of oxidative stress can promote autophagy in neurons, probably according to a mechanism that involves the Nrf2 pathway, as shown in Nrf2-KO mice, that instead expressed a lower number of genes related to autophagy [27]
Murray RZ, Stow JL
