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The staining was followed by washing twice in 1X Perm Wash and fixed with 1.6% paraformaldehyde (Thermo Fisher) for 10 min at RT

An optimal antimalarial should not elicit resistance and should neutralize all parasite stages inside the human host, namely (i) hepatic forms associated to the asymptomatic onset of disease, (ii) dormant liver forms (hypnozoites) responsible for relapse in certain malaria types, (iii) blood-stage forms underlying the symptomatic phase of infection, and (iv) gametocytes, responsible for host-to-vector transmission

Twisted epithelial-mesenchymal transition blocks senescence