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Keywords: tumor resistance, glutathione S -transferase pi, drug treatment Introduction In addition to combating a variety of noxious substances from the external environment, cell-detoxification mechanisms are capable of resisting the deleterious effects of certain endogenous substances (eg, ROS, a product generated from normal cellular metabolism), in order to maintain physiological homeostasis

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but, at the high dose (8 mg/kg/day), most of the animals succumbed by the end of the first week of treatment and others that survived presented signs of peritonitis [329]

Therefore, it is important to exactly understand the effects of injection factors that influence the permeation and diffusion of the drug in subcutaneous tissue