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Pathophysiological role of purines and pyrimidines in neurodevelopment: unveiling new pharmacological approaches to congenital brain diseases

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Eluted proteins were concentrated using Amicon Ultra centrifugal filters (10 kDa or 30 kDa MWCO) and further purified by size-exclusion chromatography on a HiLoad 16/600 Superdex 200 prep-grade column (Cytiva) equilibrated with 20 mM HEPES (pH 7.5) and 150 mM NaCl