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Cell culture Primary cultured neurons and astrocytes were prepared from the mesencephalon and striata, respectively, of SD rat embryos at 15 days of gestation using a method described previously 33

HCT116 cells were cultured in McCoys 5A medium (Wisent, Nanjing, China) supplemented with 10% fetal bovine serum (Gibco, Carlsbad, CA), 100 g/ml streptomycin, and 100 units/ml penicillin G in 5% CO 2 at 37C and subcultured by trypsinization when confluent

Benefits of GHK-Cu - What This Copper Peptide Can Do and What the Science Actually Supports By: Marc Lobliner, IFBB Pro GHK-Cu is one of the few peptides that stands up to real scrutiny