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Description
Prior to in-solution enzymatic digestion with trypsin overnight at 25C (1:50 trypsin:protein ratio), sample solutions were evaporated to half of the starting volume by vacuum centrifugation to increase protein concentration maintaining SDC at a concentration tolerable for trypsin activity

Sun C Shen L Zhang Z and Xie X

AP2/ERF Transcription Factors and Their Functions in Arabidopsis Responses to Abiotic Stresses

Science 312 , 12111214 (2006)
