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Notably, traditional NHS-mediated maleimide functionalisation leads to a maximum in reaction yield of 70% due to constant maleimide hydrolysis (see Supplementary Fig

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Similarly, interference with NADPH oxidases, the primary ROS-generating enzymes, and mitogen-activated protein kinases (MAPKs), key mediators of ROS signaling, leads to cytoskeletal disorganization, emphasizing the critical requirement of redox balance during cell division (Mhamdi and Van Breusegem, 2018)