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Enzyme-linked immunosorbent assay (ELISA) Spinal cord tissue and muscle tissue were weighted and quickly homogenized in ice cold PBS according to manufactures protocols followed by centrifugation at 4500 g for 5 min at 4 C

A randomized placebo-controlled trial involving intravenous infusion of allogeneic adipose-derived MSCs reported no significant improvement in serum creatinine levels, although an upward trend in quality-of-life indicators was observed (129)

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This process coincides with the initiation of pyroptosis, facilitating the release of IL-1 into the extracellular environment