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23 The calibration curve for vitamin B 12 was prepared using five points in the range of 0.0103-0.33 mg/ml, and the regression parameter was equal to 1.For determination of the accuracy and precision of the method four samples with concentrations in calibration range were used

A subcutaneous injection uses a shorter needle to inject the B12 solution into the tissue layer between the skin and muscle, but it may be a slower absorption rate than other methods of administration