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The reverse transcription quantitative polymerase chain reaction (RT-qPCR) cycling conditions were set as follows: reverse transcription at 50C for 30 min, pre-denaturation at 95C for 2 min, followed by 40 cycles of denaturation at 95C for 15 s and annealing/extension at 60C for 1530 s

Between the zygote and 2-cell stages of mouse embryogenesis, maternally derived miRNAs are globally degraded

A lot of that confusion clears up when you understand what's happening at the cellular level

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