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These limitations do not invalidate preclinical findings but emphasize the need for human clinical trials with appropriate sample sizes, standardized protocols, and validated outcome measures before establishing clinical efficacy in human patients

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Published preclinical studies used intraperitoneal injection in rodents, which bypasses the subcutaneous absorption variability human protocols face

Vaginal Tightening procedures can reverse these effects by tightening and firming the vaginal canal, restoring it to a more youthful state, and improving overall vaginal health