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Not including these can slow down results

Phytomedicine 135, 156117
Archer, B., Ueberrueck, T., Mack, J., Yousef, K., Jarenwattananon, N.N., Rall, D., Wypysek, D., Wiese, M., Bluemich, B., Wessling, M., Iruela-Arispe, L., and Bouchard, L., "Non-Invasive Quantification of Cell Density in 3D Gels by MRI", IEEE Trans Biomed Eng 1-10 (2018)

mRNA real-time PCR RNA was extracted from epSPCs using TRIzol (Thermo Fisher Scientific) according to manufacturers protocol