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J.MariniB.Gov'tNon-U

E.FanS.Durbin-JohnsonB

People with severe kidney/liver issues, allergies to components, or pregnant individuals should consult a doctor before starting

In short, genomic DNA was extracted by cell lysis (10 mM Tris-HCl, 10 mM EDTA, 150 mM NaCl and 0.1 % SDS), proteinase K treatment (New England Biolabs) and DNAse-free RNAse digest (Thermo Fisher), followed by two rounds of phenol extraction and isopropanol precipitation