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Inactivation of the GSTA4 Gene Inactivation of the GSTA4 gene was carried out using the lentiCRISPRv2 system (Addgene, MA, USA) (24)
Materials and methods Cell culturing RWPE-1 (ATCC CRL-11609) epithelial cells and DU-145 (ATCC HTB-81) human prostate carcinoma cells were purchased from American Type Culture Collection (Manassas, Virginia, USA)
The results were in agreement with another report indicating that only DHLA effectively inhibited Cu(II)-dependent ascorbate oxidation [37]
