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10.1097/MCG.0b013e31819f5652 69
RNA Extraction and RT-PCR in the Tendon-Muscle Junction Samples At 7, 14, 28, and 42 postoperative days, TRIzol Reagent (Thermo Scientific, Waltham, MA, USA) was used for extraction, and spectrophotometer (NanoDrop, Thermo Scientific) for quantification at 260 nm of the tissue RNA (Table 1)

Mistake 1: spraying water directly onto the powder This is the number one reconstitution mistake
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