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GST is also a commonly used solubility tag, meaning it is added N-terminally to another protein to help keep it soluble (Figure 2)

Feature GST Pull-Down Immunoprecipitation Binding Moiety GST Tag Antibody (Protein A/G/L) Beads Glutathione Agarose Beads Protein A/G/L Agarose Elution Harshness Mild Harsh Specificity High Varies Experimental Requirement Cloning with GST Tag Antibody availability How to do a GST pull-down

Neonatal rat cardiac myocytes were treated with GPC at 1, 10, 80, and 100 M concentrations for 15 min, 3 h, or 24 h, respectively

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