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Erastin impairs cystine uptake through system Xc, leading to decreased GSH levels and reduced GPX4 activity, which weakens cellular antioxidant defenses, increases lipid ROS and induces ferroptosis

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Cells remaining on inside membrane surface in the upper chambers were removed with a cotton swab, whereas the cells on the lower surface were fixed in methanol for 10 min at room temperature, washed with PBS and stained with 0.5% crystal violet and counted as described previously (Gupta et al