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Quantitative PCR was performed to quantify the expression levels of the tested genes

Common signs of B12 deficiency include fatigue, weakness, pale skin, shortness of breath, numbness or tingling in the hands and feet, difficulty maintaining balance, and cognitive issues like memory problems

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By integrating nitrogen metabolism with the tricarboxylic acid (TCA) cycle, NADPH-GS activity enables the incorporation of inorganic nitrogen into organic compounds, thereby promoting the synthesis of proteins and other essential metabolites (Fortunato et al