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The elution was concentrated using an Amicon Ultra Centrifugal Filter (MWCO 100 kDa) and subjected to size-exclusion chromatography (SEC) on a Superdex 200 Increase 10/300 column that was pre-equilibrated with 20 mM HEPES pH 7.4, 100 mM NaCl, 2 mM MgCl 2 , 1 M peptide, 0.01% (w/v) LMNG and 0.0006% (w/v) CHS

10.2147/DDDT.S82030 32 HunterJ

Rat tissue sections were pretreated for 15 min with HIER at 95 C in ER2 (Leica) followed by a 15-min protease treatment and then hybridized with rat-specific probes to detect mRNA transcripts for CALCR (477798), PRLH (1301288-C2) and GLP1R (315228-C3

Coenzyme Q10 in patients with endstage heart failure awaiting cardiac transplantation: A randomized, placebocontrolled study