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They are classically seen in glucose-6-phosphate dehydrogenase deficiency, where impaired glutathione production leaves red blood cells vulnerable to oxidative damage

In the GST family, early studies demonstrated that the GSTP1 gene plays an important role in several cellular processes, including catalysis and deoxylation of electrophilic compounds, oxidative stress regulation, cell signaling and carcinogenesis (25,26)

The next day, cells were collected in ice cold PBS, pelleted and sonicated in NP-40 lysis buffer (50 mM Tris pH 8, 150 mM NaCl, 5 mM EDTA, 0.5% NP-40) with protease inhibitors (Roche)

10.1093/annonc/mdx348 156 RanaI.OhJ.BaigJ.MoonJ