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The protein concentrations of the samples were determined using a Protein Assay Bicinchoninate Kit (Nacalai Tesque, Inc., Kyoto, Japan)
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They include various free radicals and reactive molecules formed from molecular oxygen and nitrogen, including superoxide anion radical (O ), hydroxyl radical (OH ), hydrogen peroxide (H 2 O 2 ), singlet oxygen ( 1 O 2 ), nitric oxide (NO), and peroxynitrite (ONOO ) [28]
