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In contrast to the enzymatic pathways, non-enzymatic H 2 S production is partially catalyzed by the synergistic action of vitamin B6 and iron, using cysteine as a substrate ( 2 S can be oxidized in mitochondria by sulfhydryl reductase (SQR) or methylated in the cytoplasm by sulfhydryl-S-methyltransferase ( 2 S is excreted through biological fluids after binding with methemoglobin or other metal and disulfide-containing molecules (Figure 2)

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