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When the pH of the muscle reaches the isoelectric point, the positive and negative charges on the active substances on the water-protein binding sites are equal, which will lead to the inability to combine with the charged groups of water and ultimately result in a decrease in the WHC of the meat 34

doi: 10.1016/j.postharvbio.2007.09.028 37 Exposito-RodriguezM.LaissueP

Opioid and/or cocaine dependence was diagnosed via interview and the DSM-IV criteria

In the UK, pernicious anaemia is typically treated with intramuscular hydroxocobalamin injections, though high-dose oral supplements may be considered in some cases under clinical supervision