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Samples were washed with PBS three times and dehydrated using a graded ethanol series (30%, 50%, 70%, 85%, 95%, and 100%), with each step lasting 15 minutes
Wiley-Balckwell, 2010
Thus, replacement of leucine 973 C terminal to the NPEY motif in the IR by phenylalanine, the residue found in the homologous region of the IGF1R, is sufficient to largely switch the cell signalling and gene expression patterns toward those of the IGF1R
Extended light exposure degrades B12 and can affect overall formulation stability
