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Central to this advancement is the innovative use of the Tiron/ferrous ion (Fe 2+ ) reagent, which simplifies the assay by eliminating the need for protein precipitation, a step that previously introduced variability

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525 Age-dependent changes of astrocytic morphology have been understudied, with some controversial observations reported when only the morphology of GFAP-stained profiles was analysed, which can easily be misinterpreted

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