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Clonal WT MEFs were generated in parallel from the same parental clonal lines, except using a plasmid expressing pSpCas9-2a-GFP and a non-targeting sgRNA (Supplementary Table 4)

mitophagy can regulate mitochondrial homeostasis and reduce ROS production, thus exerting a certain anti-apoptotic and reduced necrotic cell death effects [102]

Fasting Glucose and HbA1c: Because growth hormone naturally mobilizes fatty acids and can reduce insulin sensitivity over extended periods, monitoring blood sugar levels is vital to ensure metabolic health is maintained

Even in randomized controlled trials (RCTs), adequate blinding has proven difficult, and sample sizes have generally been small (Figure 2)