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The UVvis absorption and fluorescence emission spectra of the GSH-CDs were recorded on a multimode microplate reader (TECAN, Switzerland)
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Transfected cells were metabolically labelled with -alkynyl-palmitate, GSTP1 was immunoprecipitated using agarose beads conjugated to mouse anti-V5 antibody, then subjected to click chemistry and analyzed by western blotting with neutravidin-HRP and rabbit pAb anti-GSTP1 (Fig 3B)
