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For determining combinatorial treatment effects with either Reversan, MK-571, BSO or Ethacrynic acid, the medium was supplemented with the respective compound 24 h prior to assay preparation to impair transporter/enzyme function before the addition of the second drug

Moreover, it has been demonstrated that Idebenone can inhibit the activity of complex I through the blockade of its Coenzyme Q 10 binding pocket, hence preventing the physiological reduction of the endogenous quinone [32,33]

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Badran, M., Abuyassin, B., Golbidi, S., Ayas, N., & Laher, I