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Mol Cell Biol 29(1):2030

Two rules apply to all four

Although the etiology of IC/BPS is unclear, various factors have been implicated as possible causes, including inflammatory responses, mast cell activation, loss of the glycosaminoglycan layer of the bladder wall, urethral epithelial dysfunction, autoimmune mechanisms, infection, ischemia, and injury [2]

All cell culture and passaging was performed on the CompacT SelecT (Sartorius Stedim Biotech) automation system in barcoded, triple-layer T500 flasks (132925, ThermoFisher Scientific)