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The assay has yet to be simplified to the point that it can be carried out by routine clinical laboratories but early studies suggest that it may also predict ASCVD because of the link between SM and the propensity for LDL to aggregate (14)
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Fixed slides were incubated at 4 C overnight with primary antibodies against -catenin, E-cadherin (both from BD Biosciences), Claudin-7 (Thermo Fisher Scientific), or FLAG (Sigma)
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