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Pellets were grinded with pestles in 100 l of the same buffer, cells were lysed by two freeze-thaw cycles and insoluble material was removed by filtration in a COSTAR R Spin-X centrifuge tube filter
Circadian and developmental regulation of N -methyl-d-aspartate-receptor 1 mRNA splice variants and N -methyl-d-aspartate-receptor 3 subunit expression within the rat suprachiasmatic nucleus
