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But some plant GSTs show activity on ITCs in vitro 37,38,39 , and exogenous application of AITC upregulates transcript levels of GST s and depletes glutathione 17,40,41

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Furthermore, purified CFE systems by design contain no chaperones and extract-based systems may or may not have sufficient levels of endogenous chaperones to adequately support proper folding, and thus CFE systems are frequently supplemented with additional chaperones (e.g., DnaK/DnaJ/GrpE, GroEL/GroES)