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To construct a pTRV2- TDR4 recombinant, a 360-bp Eco RI/ Bam HI-containing DNA fragment of the TDR4 gene, corresponding to nucleotides 323682 (NM_001247244.2), was amplified from tomato fruit complementary DNA (cDNA) using primers TDR4 -VIGS-For and TDR4 -VIGS-Rev (Supplementary Table S1)

[DOI] [PubMed] [Google Scholar] 29.Sikiric P., Buljan M., Vnuk D., Krstonijevic Z., Sever M., Lojo N., Drmic D., Zenko Sever A., Baric M., Starcevic N., et al

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Hyaluronic acid fillers typically last for nine to twelve months before additional treatment is required, while Sculptra can last up to two years after a series of treatments