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The membranes were washed and incubated with the respective HRP-coupled secondary antibodies for one hour at room temperature: goat anti-rabbit IgG H&L (#ab205718, 1:5000
Vlacil AK, Bnfer S, Jacob R, Trippel N, Kuzu I, Schieffer B, et al
Unless otherwise stated, yeast was grown in YPD (1% yeast extract, 2% peptone, 2% glucose) medium at 30 C to an OD 600 of 1.5
