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The membrane was first blocked in 5% (wt./vol.) skim milk powder (Lab M Limited, Heywood, UK) in tris-buffered saline with 0.1% tween20 (TBST) for 1 h at RT, then was probed with the primary antibody (concentrations and manufacturers in Supplementary Table 4) diluted in TBST with 5% BSA for 16 h at 4 C on a rocker

Activation by ROS appears to involve oxidation of key cysteine residues in the upstream activators of NF B and in many situations is prevented by antioxidants or reducing agents (141)

Pontin arginine methylation by CARM1 is crucial for epigenetic regulation of autophagy

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