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Description
Reduction and alkylation was performed by adding Tris(2-carboxyethyl)phosphine (TCEP) and 2-Chloracetamide (CAA) at the final concentrations of 10 mM and 40 mM, respectively, and incubating them for 5 min at 45 C
10.1016/j.jaci.2007.01.016 51 Klein GeltinkR
It activates natural detox enzymes and supports hormone metabolism particularly estrogen
The extracted DNA was subsequently cleaned and concentrated using the DNA Clean & Concentrator-5 kit (Zymo Research, Cat
