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The progression of fibrosis primarily involves the regulation of multiple signaling pathways, including TGF-/Smad, MAPK, Wnt/-catenin, PI3K/Akt, JAK/STAT, and Notch (Zhang et al., 2021a

After this period, you must dispose of the vial responsibly to prevent any risk of bacterial contamination
To determine the intracellular glutamate concentrations, a Glutamine/Glutamate-Glo Assay (Promega) was used following the manufacturers instructions