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Nauser T, Schoneich C
yet, citrate is made in bulk amounts using either (i) carbons from glutamine by the process of reductive carboxylation of -ketoglutarate 98,282,283 (Fig
The reaction buffer consisted of RB + BSA with 1 U/ml hexokinase, 2.5 mM d-glucose, 2.5 U/ml G-6-PDH, 2.5 mM NADP + , 10 mM succinate, 10 mM glutamate, 0.5 mM malate, and 10 M cytochrome c (all reagents from Sigma)
A test to check for the ideal 'acid to base' ratio to minimize irritation caused by the injection itself
