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Vigorous rubbing of the treated area may negatively affect your results
However, previous publications on vitamin B12 and NAFLD usually contained a small number of subjects and did not consider covariates in univariate analysis
Nutrition Reviews, 76(10), pp.778792

Common experiences after the first injection include: Mild soreness or redness at the injection site (usually resolves within 12 days) Slight improvement in energy levels or mood No immediate noticeable change (particularly common if deficiency is severe) Rarely, mild side effects such as headache or dizziness Some patients report their urine becoming brighter or more intensely coloured following injection, which occurs because excess B12 is excreted renally
