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The morphological features of ferroptosis primarily involve mitochondrial alterations including rupture of the mitochondrial membrane, reduced mitochondrial size with increased mitochondrial membrane densities, and loss of mitochondrial cristae (Li J
However, this strategy also has certain limitations
After staining the nuclei with DAPI for 5 min, observations were performed using a confocal laser scanning microscope (CLSM) on cells that were sealed with a microscope glass slide
