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Solutions: Wash buffer: 50 mM Tris, 5 mM EDTA, 1 g/ml leupeptin, 1 g/ml pepstatin, 0.15 mm PMSF, pH 8.0 U buffer: 5 M urea, 50 mM Tris, 5 mM EDTA,5 mM 2-ME, 1 g/ml leupeptin, 1 g/ml pepstatin, 0.15 mM PMSF, 1 mM DFP pH 8.0 PBS/glycerol: 1X PBS, 20% glycerol, 1% Triton X-100, 5 mM 2-ME, 5 mM EDTA, 5 mM 2- ME, 1 g/ml leupeptin, 1 g/ml pepstatin, 0.15 mM PMSF, 1 mM DFP pH 7.4 21 Use of a peristaltic pump is advised to evenly control flow rates

This risk is observed for up to 14 days after the injection

Sugar in your bottle will allow even more bacteria to grow, she says