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Description
Slices were cultured in Minimum Essential Medium (MEM) with Hanks balanced salts [Thermo Fisher Scientific, #11575032], 1% penicillinstreptomycin and 25% horse serum [Thermo Fisher Scientific, #16050130] for 3 days, with 1 mL of medium added below the membrane insert

they're temporarily starved of the resources they need

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CoQ10 has a favorable safety and tolerability profile at recommended dosages (typically around 100 mg/day), with studies indicating no significant adverse effects even following prolonged exposure to doses as high as 900 mg/day (171)
